VirtualBox: The image file is inaccessible and is being ignored

The image file ‘E:\Downloads\ubuntu-16.04-desktop-i386.iso’ is inaccessible and is being ignored. Please select a different image file for the virtual DVD drive..

I mounted my ubuntu .iso file. When I started the new and blank VirtualBox VM, I selected the mounted drive as the start up drive. The booting crashed with the above given error.

I dismounted it. I removed the virtual optical drive in the VM settings. Then I pointed the .iso file directly as DVD drive to resolve this issue.

Oracle Virtual Box

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